CIFT

Real time PCR

               

 

Make

Thermofischer scientific, USA 

Model

7500 Realtime PCR system

 

 

 

  •  
Capacity: 1 x 96-well plate (Fast), 12 x 8-tube strips; Reaction Volume Range: 10-30 µl
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Sensitivity: (Concentric Nebuliser) better than 109 counts per seconds for 1 ppm of In
  •  

Quantification Limit: Better than 1 ppt.

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Dynamic Range: Linear for 12 decades, i.e. 1012

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Dark Noise: Less than 0.2 counts per second.

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Signal Stability: Better than < 0.5 % RSD over 30 minutes 
Better than <1 % RSD over 1 hour.

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Mass Stability: <10 ppm over one hour 
< 20 ppm over 10 hours.

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Scan Speed: (Magnetic) 1 to 240 amu in less than 70 ms.

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Scan Speed: (Electric) less than 1 ms / jump irrespective of mass range.

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Oxides & Doubly charged Ions: BaO+ /Ba+ less than <0 .002 % 
Ba2+ / Ba+ less than <0.02 %

  •  

Radio Frequency Generator: 27 MHz with 2 KW RF Power with automatic Reflected power matching network and fully protected against over load and heating. RF section air - cooled and load coil water cooled with its own standalone heat exchanger.

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Plasma Torch: Full demountable torch with sample tube (ceramic / Sapphire injector) suitable for HF based solutions and additional injector for organic solvents. Computer controlled three Argon Mass Flow Controller for torch and nebuliser.

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Nebuliser: Concentric Nebulizer for normal acid based solution with flow rate of 50 micro-litre / min and 100 micro-litre / min. Suitable Nebuliser for HF based solution with cyclonic spray chamber.

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Spray Chambers: 
1. Dual Cyclone double pass Quartz Spray chamber for high accuracy measurement compatible with Peltier cooling. 
2. PFA Spray chamber for ultra trace analysis and HF based solutions. 
3. Peristaltic Pump: For constant (regulated) sample flow and drain.

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Peltier Cooling System: Fully Computer controlled Peltier cooling system for accurate temperature control with quartz / PFA Spray chamber.

 

 

The 7500 software v2.0 for the 7500 Fast and 7500 Real-time PCR Systems runs on the Windows® XP and Microsoft Vista OS operating systems. Flexible five-color detection systems are easily calibrated for various dyes without requiring the addition of new filter sets. Variable excitation capacity allows greater sensitivity for the greatest range of dyes.A real-time polymerase chainreaction (Real-Time PCR), also known asquantitative polymerase chain reaction(qPCR), theamplification of a targeted DNA moleculeduring the PCR are monitored on a real time.

 

 

 

Provide the type of experiment to be performed on the sample given. Primer and target DNA details and type of the assay should be included. Cycling condition of the assay should be given

 

 

 

Industry

University

National Lab/R&D's

 

Standardization and entire setup (User provides details of primers, dye and template in Pure form)

2000 per reaction

500 per reaction

1000 reaction

Per sample

Only Plate set up (User provides primers, dye and template in pure form)

600 per reaction

200 per reaction

400 per reaction

Per element/sample

 

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