Pulsed field gel electrophoresis



Biorad, USA


CHEF Mapper® XA system, 2012CHEF Mapper® XA system, 2012





CHEF Mapper system has the chamber, power module, variable speed pump and cooling Module.


System has the embedded algorithm for automated optimization of common electrophoresis conditions for smallest and largest size DNA (range 1 kb to 6 mb)


Power module having power supply of 350V, gradient of continuously adjustable 9V/cm, with 0, and 0.6–9 V/cm, in 0.1 V/cm increments


20 programs can be stored


Switching of power supply can be done from 50 msec to 18 hr, with an angle variation of 0–360 degrees and an increments of in 0.5° increments


Electrophoresis cell has the facility of 24 electrodes of platinum and mounted with temperature probe


Other accessories includes such as variable speed oscillating pump 120 V, ground isolated. Flow rate 1 liter/min, casting stand 14 cm x 13 cm, comb 15 well comb and comb holder

Tygon tubing 365 cm, Disposable Sample plug mold 50 slot


Cooling module includes 75 watts of input power at 14 °C and range of 5–25 °C



Pulsed field electrophoresis is a powerful technique for resolving chromosomal sized DNAs. Alternating the electric field between spatially distinct pairs of electrodes causes megabase (mb) size DNAs to re-orient and move at different speeds through the pores in an agarose gel. The CHEF Mapper system separates large and small DNA fragments with better resolution, speed, and accuracy than traditional pulsed field methods. DNAs ranging from 100 bases to over 10 megabases may be effectively resolved. The system provides highly uniform, or homogeneous, electric fields within the gel, using an array of 24 electrodes, some of which are clamped, or held to intermediate potentials to eliminate lane distortion. PFGE analysis can be used in epidemiological investigation, detection of outbreak-related cases, distinguishing outbreaks strains from other strains, and tracking contamination within a food system.  It is considered as"gold-standard" for subtyping many pathogenic organisms.




Sample should be submitted as young cultures in suitable media in agar plate or agar slant. If more than two cultures needs to be compared, send only the related species of bacteria. Send only the pure culture. If cultures sent requires special cultivation condition, please do mention it. Should provide the detail of restriction enzyme to be used for typing.





National Lab/R&D's


PFGE analysis for one isolate and one enzyme

Rs. 15000/-  Per Isolate

Rs. 3500/-  Per Isolate

Rs 7000 /-  Per Isolate


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